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primescripttm strand cdna synthesis kit rt master mix  (TaKaRa)


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    Structured Review

    TaKaRa primescripttm strand cdna synthesis kit rt master mix
    Genetic analysis and expression of SURF1 gene variants in Case 2. (A) The probands 2 (P2) intronic variant c.515 + 3G > C caused abnormal splicing of the SURF1 gene, leading to the deletion of exon 5. (B) The expression level of the SURF1 gene in probands 2 (P2) was significantly lower than those in their parents. (C) PCR results of <t>cDNA</t> from the nuclear family of probands 2 (P2), the amplicon in the proband was shorter than the corresponding bands in the parents.
    Primescripttm Strand Cdna Synthesis Kit Rt Master Mix, supplied by TaKaRa, used in various techniques. Bioz Stars score: 96/100, based on 1482 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/primescripttm+strand+cdna+synthesis+kit+rt+master+mix/pmc13083003-55-26-33?v=TaKaRa
    Average 96 stars, based on 1482 article reviews
    primescripttm strand cdna synthesis kit rt master mix - by Bioz Stars, 2026-07
    96/100 stars

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    1) Product Images from "Study on Leigh syndrome caused by SURF1 gene mutations and its mechanisms"

    Article Title: Study on Leigh syndrome caused by SURF1 gene mutations and its mechanisms

    Journal: Frontiers in Neurology

    doi: 10.3389/fneur.2026.1793054

    Genetic analysis and expression of SURF1 gene variants in Case 2. (A) The probands 2 (P2) intronic variant c.515 + 3G > C caused abnormal splicing of the SURF1 gene, leading to the deletion of exon 5. (B) The expression level of the SURF1 gene in probands 2 (P2) was significantly lower than those in their parents. (C) PCR results of cDNA from the nuclear family of probands 2 (P2), the amplicon in the proband was shorter than the corresponding bands in the parents.
    Figure Legend Snippet: Genetic analysis and expression of SURF1 gene variants in Case 2. (A) The probands 2 (P2) intronic variant c.515 + 3G > C caused abnormal splicing of the SURF1 gene, leading to the deletion of exon 5. (B) The expression level of the SURF1 gene in probands 2 (P2) was significantly lower than those in their parents. (C) PCR results of cDNA from the nuclear family of probands 2 (P2), the amplicon in the proband was shorter than the corresponding bands in the parents.

    Techniques Used: Expressing, Variant Assay, Amplification



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    Genetic analysis and expression of SURF1 gene variants in Case 2. (A) The probands 2 (P2) intronic variant c.515 + 3G > C caused abnormal splicing of the SURF1 gene, leading to the deletion of exon 5. (B) The expression level of the SURF1 gene in probands 2 (P2) was significantly lower than those in their parents. (C) PCR results of <t>cDNA</t> from the nuclear family of probands 2 (P2), the amplicon in the proband was shorter than the corresponding bands in the parents.
    Primescripttm Strand Cdna Synthesis Kit Rt Master Mix, supplied by TaKaRa, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/primescripttm+strand+cdna+synthesis+kit+rt+master+mix/pmc13083003-55-26-33?v=TaKaRa
    Average 96 stars, based on 1 article reviews
    primescripttm strand cdna synthesis kit rt master mix - by Bioz Stars, 2026-07
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    primescripttm rt master mix  (TaKaRa)
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    Genetic analysis and expression of SURF1 gene variants in Case 2. (A) The probands 2 (P2) intronic variant c.515 + 3G > C caused abnormal splicing of the SURF1 gene, leading to the deletion of exon 5. (B) The expression level of the SURF1 gene in probands 2 (P2) was significantly lower than those in their parents. (C) PCR results of <t>cDNA</t> from the nuclear family of probands 2 (P2), the amplicon in the proband was shorter than the corresponding bands in the parents.
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    primescripttm rt master mix 1st strand cdna synthesis kit  (TaKaRa)
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    Genetic analysis and expression of SURF1 gene variants in Case 2. (A) The probands 2 (P2) intronic variant c.515 + 3G > C caused abnormal splicing of the SURF1 gene, leading to the deletion of exon 5. (B) The expression level of the SURF1 gene in probands 2 (P2) was significantly lower than those in their parents. (C) PCR results of <t>cDNA</t> from the nuclear family of probands 2 (P2), the amplicon in the proband was shorter than the corresponding bands in the parents.
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    Image Search Results


    Genetic analysis and expression of SURF1 gene variants in Case 2. (A) The probands 2 (P2) intronic variant c.515 + 3G > C caused abnormal splicing of the SURF1 gene, leading to the deletion of exon 5. (B) The expression level of the SURF1 gene in probands 2 (P2) was significantly lower than those in their parents. (C) PCR results of cDNA from the nuclear family of probands 2 (P2), the amplicon in the proband was shorter than the corresponding bands in the parents.

    Journal: Frontiers in Neurology

    Article Title: Study on Leigh syndrome caused by SURF1 gene mutations and its mechanisms

    doi: 10.3389/fneur.2026.1793054

    Figure Lengend Snippet: Genetic analysis and expression of SURF1 gene variants in Case 2. (A) The probands 2 (P2) intronic variant c.515 + 3G > C caused abnormal splicing of the SURF1 gene, leading to the deletion of exon 5. (B) The expression level of the SURF1 gene in probands 2 (P2) was significantly lower than those in their parents. (C) PCR results of cDNA from the nuclear family of probands 2 (P2), the amplicon in the proband was shorter than the corresponding bands in the parents.

    Article Snippet: Total RNA was extracted from patients and their parents using the Qiagen RNA Preparation Kit, and complementary DNA (cDNA) was synthesized by reverse transcription using the PrimeScriptTM Strand cDNA Synthesis Kit/RT Master Mix (TAKARA).

    Techniques: Expressing, Variant Assay, Amplification